ANÁLISE DO POLIMORFISMO GENÉTICO DO CÓDON 72 DO GENE P53 EM PACIENTES COM CARCINOMA ESCAMOSO DE BASE DA LÍNGUA

Abstract

INTRODUCTION: The polymorphism at codon 72, proline (p53P) or arginine (p53R) is involved in the ability of p53 to interact with cellular proteins. Several authors have shown that the presence of genotype p53RR confers greater risk of developing tumors. OBJETIVE: To assess the allelic frequency of genetic polymorphism in codon 72 in TP53 gene in samples obtained from patients diagnosed with squamous carcinoma of base of the tongue treated at the Hospital Araújo Jorge and Associação de Combate ao Câncer em Goiás (ACCG)between 1990 and 2006. Evaluate the genetic predisposition for Base of Tongue Cancer linked of TP53 polimorphism, by identififyin of the presence or absence of the alleles p53R and/or p53P alleles in patients with this pathology. MATERIALS AND METHODS: This study is a casecontrol retrospective in nature, was conducted at Núcleo de Pesquisas Replicon of the Universidade Católica de Goiás in conjunction with the Hospital Araújo Jorge. We evaluated 54 patients with squamous carcinoma of the base of the tongue and 186 individuals without cancer. These were matched regarding gender, age, and the group of cases was evaluated clinical stage and smoking, alcohol consumption. The genotypes p53R and p53P were determined by PCR, using specific primers. RESULTS: The allele frequencies for p53R in cases and controls were 75.9% and 74.2%, while the p53P were 24.1% and 25.8% respectively. There was no statistically significant difference (p = 0.79) in allele frequencies between the two groups, suggesting that the polymorphism of codon 72 of TP53 is not a risk factor for susceptibility to squamous cell carcinoma of the tongue base. CONCLUSION: The study does not find relationship between p53R and CCO carcinogenesis when compared sex, age and color.