AVALIAÇÃO MOLECULAR POR ARMS-PCR DO SNP -1082 G/A DA IL-10 NA DOENÇA PERIODONTAL CRÔNICA EM ADULTOS

Abstract

Periodontitis is considered an inflammatory disorder of bacterial etiology that results in damage to the periodontal tissue, due to complex interactions between periodontal pathogens and host defense system. Genetic mechanisms may modulate the response of an individual because they can interfere with gene expression of important mediators of inflammation. The objective of this study was to evaluate the association of the polymorphism of SNP-1082G / A gene promoter in interleukin-10 with periodontal disease in 36 cases and 30 controls. Strategy was used ARMS-PCR for allelic discrimination. Of individuals with periodontal disease, 16 (44%) had genotype AG, followed by 13 (36%) with genotype GG and 7 (20%) with genotype AA, highlighting a greater prevalence of heterozygotes for locus of IL-10, especially in individuals aged from 30 to 35 years in the control group, 13 (43%) of subjects had the genotype AG, 12 (40%) had GG and only 5 (17%) were classified as AA, highlighting a higher prevalence of heterozygotes for locus of IL-10, especially in individuals aged from 40 to 45 years. The data indicated that the study populations were in equilibrium according to Hardy-Weinberg Theorem. The analysis of the frequency of genotypes and allele frequencies concluded that no causal relationship found between the presence of genotype G or A and the development of periodontal disease in adults. The SNP-1082G / A IL-10 gene showed no predictive value for periodontal disease and therefore can not use it with prognostic value.