AVALIAÇÃO DO EXTRATO ETANÓLICO BRUTO DAS CASCAS DO CAULE DA CROTON URUCURANA BAILL NA CICATRIZAÇÃO DE FERIDAS CUTÂNEAS INDUZIDAS POR PEÇONHA DE BOTHROPS MOOJENI EM CAMUNDONGOS.

Abstract

INTRODUCTION: The herbal and other natural products are widely used therapeutic resources to aid the healing of skin wounds of early civilization to the present. Among the plants used for treatment of infected wounds, the Croton urucurana stands out for being a healing plant that accelerates the healing process. OBJECTIVE: To evaluate the healing activity of extracts of the stem bark of Croton urucurana skin wounds in mice, induced by Bothrops moojeni. METHODOLOGY: The ethanolic extract from the stem bark of Croton urucurana (EECC) obtained through a maceration dynamic process, was diluted in saline to obtain concentrations of 10%, 20% and 40%. It was stablished a pilot experiment to determine the minimum dose necrotizing using mice (n = 5) of Swiss lineage, males with body mass index ranging from 18 to 25g. To evaluate the healing activity, it was performed in mice (n = 60) of the same type and with the same characteristics were randomly divided into five groups of twelve animals each (n = 12): GC - Control Group, GS - Group Soro, GT1 - Group Treatment with 10% EECC, GT2 - Group Treatment EECC to 20% and GT3 - Group Treatment EECC to 40%. For induction of the wound, the animals were anesthetized intramuscularly with sodium thiopental at the dose of 40mg/kg. After shaving the dorsum of the mice,it was inoculated intradermally 120μg/50µL of saline in order to obtain a necrose. RESULTS: The minimum necrotic dose of venom from Bothrops moojeni in mice is 120 mg of venom diluted into 50 uL intradermally saline in the dorsal region. There was a significant decrease of skin wounds of the GT1 group in 7th and 12th days compared to the GC. There was a significant decrease of skin wounds of the group GT2 and GT3 on the 12th day compared to the GC.The Histological analysis of the groups GT1, GT2 and GT3 allows to conclude that the skin wounds show a process of evident scarring at 12 days of treatment compared to the GC, by stimulating angiogenesis, neoformation of hair follicles and fibrous repair. CONCLUSION: The skin wounds in mice induced by the venom of Bothrops moojeni when treated with EECC (10%, 20% and 40%), have a more rapid healing process in the control group.