RELAÇÃO DE RESULTADOS SOROLÓGICOS DE ENSAIO IMUNOENZIMÁTICO (ELISA) COM A TÉCNICA DE IMUNOFLUORESCÊNCIA MICROSCÓPICA DIGITAL PARA PESQUISA DE ANTIGLOBULINA IGG DE TOXOPLASMA GONDII

Abstract

Toxoplasma gondii is an obligatory intracellular parasite, capable of infecting a large variety of intermediate hosts including humans. In the domiciliary environment, the infected final hosts (cats) spread the oocists by their excrements, which quickly reach maturity in the ground and become infectants. Therefore, toxoplasmosis is acquired by humans through the ingestion of oocists found in the domiciliary environment or by eating insufficiently cooked meat, infected with cysts. The antitoxoplasma antibodies presence can be determined by several serologic reactions, using different serologic markers to distinguish recent infection from toxoplasma-illness. In this work, we carried out serologic assays, defined a curve by the digital recognition of color using the Toxo IgG Digital Microscopical Immunofluorescence. The curve was related with the results in UI/ml read by the quantitative determination of IgG antibodies for the Toxoplasma gondii of Toxo in the G VIDAS® system. The existing correlation between sera in UI/ml and their values found through the digitalized green intensity curve was evidenced. In the analysis between the predetermined sera in UI/ml and its read values, did not show differences that could affect the laboratorial diagnosis. The analysis of the behavior of the curves defined by this works, shows that the approach adopted by this work is promising. for the development of a novel method for Immunofluorescence analysis with significant gains in precision and price reduction. The rational use of technologies, even so the rationality is not neutral, implies the election of technologies to be financed and the identification of the conditions or sub-groups where they will have to be used, in the direction to become the system of more efficient health for the objective to protect and to recoup the health of the population. Correlation to the unemployment level social impact in companies affected by the technological modernization progress. The expectation is that it has, through public politics in set with private initiative, action for creation of courses that prepare the workers for the use of these new technologies.