AVALIAÇÃO MOLECULAR POR ARMS-PCR DO SNP-1082 G/A DA IL-10 EM ASSOCIAÇÃO COM OBESIDADE INFANTO JUVENIL

Abstract

Obesity represents a global public health problem, being a disorder influenced by genetic and environmental factors. It is an important cause of morbidity and mortality worldwide and the disease characteristics, has been understood as a chronic disorder of inflammatory nature. Several studies of polymorphisms associated with the metabolic syndrome, has been performed in obese children and adolescents, demonstrating significant relationship with this pathology. The several inflammatory cytokines exert significant influence in the development and maintenance of obesity for her role in the regulation of energy homeostasis and metabolism. The aim of this study was to evaluate the association of the polymorphism of SNP 1082G / A in the interleukin-10 gene promoter with the Children and Youth obesity in 48 cases and 45 controls, associated with the lipid profile. In this study case-control was used to ARMS-PCR methodology for allelic determination. Of obese individuals, 27 (56%) had the genotype A / G, followed by 13 (27%) with genotype A / A and 8 (17%) with genotype G / G. Of the analyzed controls, 28 (62%) had Genotype A / G, followed by 9 (20%) with genotype A / A and 8 (18%) with genotype G / G. In the analysis it was found that the presence of the SNP-1082G / A polymorphism was similar in both groups, no representing risk factor to obesity. Also there was a significant difference in HDL cholesterol levels in both groups, linking obesity to low levels of HDL in adolescents and reforcing the relevance of this parameter.