O Carcinoma Colorretal (CCR) e o Papilomavírus Humano (HPV): Detecção e Genotipagem

Abstract

The incidence of colorectal cancer (CRC) is increasing worldwide and the disease is the fourth leading cause of cancer death. The association of human papillomavirus (HPV) with CRC is inconclusive and the presence of the viral genome in the CRC is the first step in elucidating this issue. The objective of this study was to retrospectively analyze 92 cases of CRC and to investigate the presence of HPV DNA, genotypes and possible associations between HPV and the clinical and pathological characteristics of CRC. It is a retrospective study that included 92 patients with CRCs assisted at a reference institution in the Center-West of Brazil. Tumor samples fixed in formalin and embedded in paraffin were used. HPV detection was performed by PCR using SPF 10 consensus primers and the commercial INNOLIPA HPV Genotyping Extra II © kit (FujirebioEurope, Ghent, Belgium). The associations between the presence of the virus and the investigated tumor characteristics were assessed by Fisher exact test, with a significance level of 5% (p <0.05). The prevalence of HPV in the CRC group was 35,8% (IC95%; 26,8-46,0). Associations between the presence of HPV and the clinicopathological aspects of CRC were not detected in this study. Our results demonstrated the prevalence of HPV DNA in a grup CRC and the absence of association between the presence of the virus and the characteristics of the tumors.