Avaliação das atividades angiogênica, antiangiogênica, mutagênica e antimutagênica da quina-do-cerrado (Strychnos pseudoquina)

Abstract

Strychnos pseudoquina is a plant widely used in traditional medicine, through the use of its leaves and stem bark in the treatment of diseases such as intermittent fevers, in the treatment of malaria, liver and stomach problems and anemia. Despite the beneficial activities, most species of the genus Strychnos are toxic and have limited data in the literature. Therefore, the present study aimed to investigate the possible angiogenic and antiangiogenic activities of Strychnos pseudoquina by carrying out “in vivo” laboratory tests, using the chorioallantoic membrane of the embryonated chicken egg as an experimental model; to investigate the mutagenic and antimutagenic potential of Strychnos pseudoquina by performing the Ames mutagenicity test with Salmonella typhimurium strain TA100. For the accomplishment of the angiogenesis tests, three concentrations of the aqueous solution of the bark of Strychnos pseudoquina were used: 15mg/mL, 30mg/mL and 60 mg/mL. The concentrations of 15mg/mL, 30mg/mL and 60mg/mL presented an average of 15.4%, 17.1% and 17.2% of vascularization in the proper order, showing a significant difference (p<0.05) in relation to the negative control and inhibitor, which presented an average of 8.9% and 2.2%, respectively. For the assessment of mutagenicity, S. pseudoquina concentrations of 10mg/plate, 5mg/plate, 2.5mg/plate, 1.25mg/plate and 0.62mg/plate, showed no significant difference (p>0.05) compared to the negative control. In the antimutagenic analysis, the concentrations of S. pseudoquina treated with sodium azide showed no significant difference (p>0.05) in relation to the positive control. It was concluded that the aqueous solution of S. pseudoquina showed angiogenic activity at concentrations of 30mg/mL and 60mg/mL and did not present mutagenic and antimutagenic action at the concentrations tested with the TA100 strain