Avaliação do SNP rs1143634 (+3954) do gene IL1B em indivíduos com a doença periodontal crônica.

Abstract

Among the many problems that affect oral health, chronic periodontal disease is the most commonly that affects populations, reaching global proportions. It is characterized by a multifactorial pathogenesis presenting, in its progression, inflammation events and destruction of protective and supporting tissues of dental elements. Interleukin-1β is a cytokine mediator of inflammatory response and contributes to the pathogenesis of chronic periodontal disease, which is characterized by bone resorption process. It is believed that single nucleotide polymorphisms (SNPs) may be associated with the progression and severity of the disease, showing discriminative phenotypes. The aim of this study was to investigate the relationship of SNP rs1143634 position +3954 C T of IL1B (IL-1β) gene with chronic periodontal disease. In the present study, we evaluated 51 individuals, divided into two groups, 26 individuals with chronic periodontal disease and 25 healthy individuals. Blood samples were obtained by venipuncture and stored in tubes with EDTA for DNA extraction from whole blood. The extracted DNA was subjected to PCR technique to amplify the region of interest, and subsequently treated with specific restriction enzyme for cleavage and achievement of fragments aiming genotyping. The data were submitted initially to the Hardy-Weinberg equilibrium Test and then the other tests, such as Chi-Square, Odds Ratio and Relative Risk, all of the 5% significance level. The genotypes were demonstrated in agreement with the hypothesis of Hardy-Weinberg equilibrium. No significant differences were observed (p> 0.05) concerning the genotype distribution and allele between groups. The presence of the C allele polymorphism rs1443634 referring to reduce significantly (p <0.05) relative risk, especially in males, while the T allele increases the risk of chronic periodontal disease.